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Substrate solution is then added and color develops in proportion to the amount of Human I-TAC
And then biotinylated Human CASP6 Antibody is added and binds to CASP6 in the sample
DDC present in the sample is added and binds to antibodies coated on the wells
The OD value is proportional to the concentration of Human IL-35
Human LCN12 (Epididymal-specific Lipocalin-12) ELISA Kit GDF Substrate solution is then addedThe Human (LCN12) Epididymal specific Lipocalin 12 ELISA Kit measures Epididymal Specific Lipocalin 12 in samples. The plate has been pre coated with Human LCN12 antibody. LCN12 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LCN12 Antibody is added and binds to LCN12 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LCN12 antibody. After incubation unbound Streptavidin
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