P1h (AggFluor Halotag Ligand) Size:100nmol 386 SMILES BrCCOCCOCCOCCCC1=C2CN(C3CCC(=O)NC3=O)C(=O)C2=CC=C1 Purity (HPLC)

386 SMILES BrCCOCCOCCOCCCC1=C2CN(C3CCC(=O)NC3=O)C(=O)C2=CC=C1 Purity (HPLC) >= 95% Shipping Conditions Room temperature Storage Conditions Refrigerated Shelf Life 12 months after the date of delivery Regulatory Statement For Research Use Only

232 SMILES OC1=C2C(=O)N(C3CCC(=O)NC3=O)C(=O)C2=CC=C1 Purity (HPLC) >= 95% Shipping Conditions Room temperature Storage Conditions Refrigerated Shelf Life 12 months after the date of delivery Regulatory Statement For Research Use Only

139 Purity (HPLC) >= 95% Solubility DMSO

trisulfo CAS Number: N/A Molecular Formula: C38H46N4O12S3 Molecular Weight: 846

P1h (AggFluor Halotag Ligand) Size:100nmol 386 SMILES BrCCOCCOCCOCCCC1=C2CN(C3CCC(=O)NC3=O)C(=O)C2=CC=C1 Purity (HPLC)P1h is a HaloTag ligand incorporating the fluorogenic AggFluor P1 dye. AggFluor is derived from the synthetic GFP chromophore, 4 hydroxybenzylidene imidazolinone (HBI), and belongs to the class of molecular rotor fluorophores widely used in biological imaging due to their sensitivity to environmental factors such as viscosity. When bound to properly folded proteins, AggFluors are quenched and remain non fluorescent; however, protein misfolding or