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And then biotinylated Mouse JAM3 Antibody is added and binds to JAM3 in the sample
Substrate solution is then added and color develops in proportion to the amount of Mouse DPP4
then added a HRP-conjugated antibody specific to Human 25-OH-D to each microplate well and incubated
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CFH
Rat IgM (Immunoglobulin M) ELISA Kit Packaging:96 wells And then biotinylated Mouse JAM3The Rat (IgM) Immunoglobulin M ELISA Kit measures Immunoglobulin M in Rat samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and
