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The concentration of TNFSF12 in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human PYY and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Substrate solution is then added and color develops in proportion to the amount of Human MAP4K4
The concentration of Rat IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve
Human LAMP1 (Lysosomal Associated Membrane Protein 1) ELISA Kit Packaging:96 wells The concentration of TNFSF12 inThe Human (LAMP1) Lysosomal Associated Membrane Protein 1 ELISA Kit measures Lysosomal Associated Membrane Protein 1 in samples. The plate has been pre coated with Human LAMP1 antibody. LAMP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LAMP1 Antibody is added and binds to LAMP1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LAMP1 antibody. After incubation unbound
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