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Then a biotinylated detection antibody specific for Mouse MIP-1Beta and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Bovine LUM Antibody is added and binds to LUM in the sample
The plate has been pre-coated with Human METAP2 antibody
Substrate solution is then added and color develops in proportion to the amount of Mouse Nox4
Human POSTN (Periostin) ELISA Kit ST16 Then a biotinylated detection antibodyThe Human (POSTN) Periostin ELISA Kit measures Periostin in samples. The plate has been pre coated with Human POSTN antibody. POSTN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human POSTN Antibody is added and binds to POSTN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated POSTN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
